1981: Organization of the Cytoplasm, Vol. XLVI
Organizer: James Watson
Part 1 Symposium Participants vForeword xiPrinciples of Organization: Functional and Spatial Order in the CytoplasmMembrane Cytoskeletal Interactions in the Human Erythrocyte D. Branton 1Regulation of Intracellular Protein Traffic G. Blobel 7Organizing the Cytoplasm for Motility F. Solomon 17Principles of Organization: Water and the Cytoplasmic ArchitectureInterrelationships between Water and Cell Metabolism in Artemia Cysts. IX. Evidence for Organization of Soluble Cytoplasmic Enzymes J. S. Clegg 23Measurements of the Diffusion of Macromolecules Injected into the Cytoplasm of Living Cells J. W. Wojcieszyn, R. A. Schiegel, and K. A. Jacobson 39Does Blebbing Reveal the Convulsive Flow of Liquid and Solutes through the Cy- toplasmic Meshwork? G. Albrecht-Buehler 45Structural Organization of the Cytoplasm M. Schliwa, J. van Blerkom, and K. B. Pryzwansky 51Cytoplasmic and Axonal FlowActive Movement of Bundles of Actin and Myosin Filaments from Muscle: A Simple Model for Cell Motility S. Higashi-Fujime 69Contraction-Relaxation Cycle of Physarum Cytoplasm: Concomitant Changes in In- traplasmodial ATP and Ca++ Concentrations N. Kamiya, Y. Yoshimoto, and F. Matsumura 77Cytoplasmic Transport: Moving Ultrastructural Elements Common to Many Cell Types Revealed by Video-enhanced Microscopy R. D. Allen, J. L. Travis, J. H. Hay- den, N. S. Allen, A. C. Breuer, and L. J. Lewis 85Fine Structure and Organization of Microfilaments in Physarum as Revealed by Chemolixation and Freeze-etching K. E. Wohlfarth-Bottermann and W. Stockem 89Cellular and Molecular Aspects of Amoeboid Movement D. L. Taylor, J. Heiple, Y.-L. Wang, E. J. Luna, L. Tanasugarn, J. Brier, J. Swanson, M. Fechheimer, P. Amato, M. Rockwell, and G. Daley 101The Axon: A Prototype for Studying Expressional Cytoplasm R. J. Lasek and S. T. Brady 113Communication between Target Organs and Nerve Cells: Retrograde Axonal Transport and Site of Action of Nerve Growth Factor M. E. Schwab, R. Heumann, and H. Thoenen 125Studies on the Mechanism of Fast Axoplasmic Transport Using Microinjection into Single Giant Neurons D. J. Goldberg, D. A. Harris, and J. H. Schwartz 135Probing Mitochondria in Living Cells with Rhodamine 123 L. B. Chen, I. C. Sum- merhayes, L. V. Johnson, M. L. Walsh, S. D. Bernal, and T. J. Lampidis 141Elements of Organization: MicrotubulesExpression of Flagellar Protein Genes during Flagellar Regeneration in Chlamydo- monas C. D. Silfiow, P. A. Lefebvre, T. W. McKeithan, J. A. Schloss, L. R. Keller, and J. L. Rosenbaum 157Autoregulatory Control of the Expression of a- and b-Tubulins: Implications for Mi- crotubule Assembly D. Cleveland and M. Kirschner 171Drosophila a-Tubulin Genes and Their Transcription Patterns L. Kalfayan, J. Loew- enberg, and P. C. Wensink 185Amino Acid Sequence of a- and b-Tubulins from Pig Brain: Heterogeneity and Regional Similarity to Muscle Proteins H. Ponstingl, E. Krauhs, M. Little, T. Kempf, R. Hofer-Warbinek, and W. Ade 191Microtubule Treadmills and Their Possible Cellular Functions L. Wilson and R. L. Margolis 199Dynein-Microtubule Interactions: ATP-sensitive Dynein Binding and the Structural Polarity of Mitotic Microtubules L. T. Haimo and B. R. Telzer 207Taxol: A New Probe for Studying the Structure and Function of Microtubules S. B. Horwitz, J. Parness, P. B. Schiff and J. J. Manfredi 219Microtubule Stability and Assembly in Living Cells: The Influence of Metabolic In- hibitors, Taxol and pH M. De Brabander, G. Geuens, R. Nuydens, R. Wille- brords, and J. De Mey 227Tubulin Assembly Sites and the Organization of Microtubule Arrays in Mammalian Cells S. L. Brenner and B. R. Brinkley 241Microtubule Structure in Caenorhabditis elegans Neurons M. Chalfie 255Asters, Poles, and Transport Properties within Spindlelike Microtubule Arrays A. S. Bajer and J. Molè-Bajer 263Approaches to Potential Sliding Mechanisms of Cytoplasmic Microtubules P. Satir 285Elements of Organization: Intermediate FilamentsDifferentiated Structural Components of the Keratinocyte H. Green, E. Fuchs, and F. Watt 293Immunolabeling Studies of Cytoskeletal Associations in Cultured Cells S. J. Singer, E. H. Ball, B. Geiger, and W.-T. Chen 303Intermediate-size Filaments: Changes in Synthesis and Distribution in Cells of the Myogenic and Neurogenic Lineages H. Holtzer, G. S. Bennett, S. J. Tapscott, J. M. Croop, and Y. Toyama 317Use of Antibodies to Intermediate Filaments in the Characterization of Human Tu- mors F. C. S. Ramaekers, J. J. G. Puts, A. Kant, O. Moesker, P. H. K. Jap, and G. P. Vooijs 331Chemistry and Biology of Neuronal and Glial Intermediate Filaments R. K. H. Liem, C. H. Keith, J. F. Leterrier, E. Trenkner, and M. L. Shelanski 341Desmin- and Vimentin-containing Filaments and Their Role in the Assembly of the Z Disk in Muscle Cells E. Lazarides, B. L. Granger, D. L. Gard, C. M. O'Connor, J. Breckler, M. Price, and 5. I. Danto 351Modification of Vimentin Polypeptides during Mitosis R. Bravo, S. J. Fey, P. Mose Larsen, and J. E. Celis 379Epithelial Tonofilaments: Investigating Their Form and Function Using Monoclonal Antibodies E. B. Lane and M. W. Klymkowsky 387In Vivo Disruption of the Cytokeratin Cytoskeleton in Cultured Epithelial Cells by Microinjection of Antikeratin: Evidence for the Presence of an Intermediate- filament-organizing Center B. S. Eckert, R. A. Daley, and L. M. Parysek 403Intermediate Filaments M. Osborn, N. Geisler, G. Shaw, G. Sharp, and K. Weber 413Differentiation-related Patterns of Expression of Proteins of Intermediate-size Fila- ments in Tissues and Cultured Cells W. W. Franke, E. Schmid, D. L. Schiller, S. Winter, E. D. Jarasch, R. Moll, H. Denk, B. W. Jackson, and K. Illmensee 431Structure of Vimentin 10-nm Filaments Probed with a Monoclonal Antibody That Recognizes a Common Antigenic Determinant on Vimentin and Tropo- myosin S. H. Blose, F. Matsumura, and J. J.-C. Liv 455Heterogeneity of Intermediate Filaments Assembled In Vitro P. Steinert, W. Idler, M. Aynardi-Whiiman, R. Zackroff, and R. D. Goldman 465Plectin: A High-molecular-weight Cytoskeletal Polypeptide Component That Copu- rifles with Intermediate Filaments of the Vimentin Type C. Wiche, H. Herrmann, F. Leichtfried, and R. Pytela 475Formation of an ATP-dependent Microtubule-Neurofilament Complex In Vitro M. S. Runge and R. C. Williams, Jr 483Part 2Elements of Organization: MicrofilamentsActin Multigene Family of Dictyostelium M. McKeown and R. A. Firtel 495Approaches for Isolating Genes Encoding the Cytoskeletal Proteins and for Reintro- ducing the Genes into Cultured Cells S. H. Hughes, J. Sorge, G. P. Thomas, J. Feramisco, L. Chow, and J. Garrels 507Actin Structure, Polymerization, and Gelation T. D. Pollard, U. Aebi, J. A. Cooper, W. E. Fowler, and P. Tseng 513How Actin Filaments Pack into Bundles D. J. DeRosier and L. G. Tilney 525Calcium-modulated Multifunctional Proteins Regulating F-Actin Organization K. Weber and J. R. Glenney, Jr 541Control of Assembly of Dictyostelium Myosin and Actin Filaments J. A. Spudich, E. R. Kuczmarski, J. D. Pardee, P. A. Simpson, K. Yamamoto, and L. Stryer 553Function of Cytoplasmic Flow in Photosensory Transduction and Phase Regulation of Contractile Activities in Physarum K. E. Wohlfarth-Bottermann and I. Block 563
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Actin Gelation and the Structure of Cortical Cytoplasm T. P. Stossel, J. H. Hartwig, H. L. Yin, K. S. Zaner, and O. I. Stendahl 569 Actinogelin: A Ca4 + -sensitive Regulatory Protein of Microfilament Organization N. Mimura and A. Asano 579 a-Actinin and Vinculin from Nonmuscle Cells: Calcium-sensitive Interactions with Actin K. Burridge and J. R. Feramisco 587 Organization of Actin Meshworks in Cultured Cells: The Leading Edge J. V. Small, G. Rinnerthaler, and H. Hinssen 599 Vinculin and a-Actinin: Interaction with Actin and Effect on Microfilament Network Formation B. M. Jockusch and G. Isenberg 613 Proteins and Complexes That Affect Actin-filament Assembly and Interactions S. Lin, J. A. Wilkins, D. H. Cribbs, M. Grumet, and D. C. Lin 625 Cell Surface: Surface Organization Partial Sequence and Turnover of Rat Liver Gap Junction Protein J.-P. Revel, B. J. Nicholson, and S. B. Yancey 633 Liver Gap Junctions and Lens Fiber Junctions: Comparative Analysis and Calmodulin Interaction E. L. Hertzberg and N. B. Gilula 639 Immunoreactive Forms of Human Erythrocyte Ankyrin Are Localized in Mitotic Structures in Cultured Cells and Are Associated with Microtubules in Brain V. Bennett and J. Davis 647 Relationships between Microfilaments, Cell-substratum Adhesion, and Fibronec- tin R. O. Hynes, A. T. Destree, and D. D. Wagner 659 Involvement of Vinculin in Contact-induced Cytoskeletal Interactions B. Geiger 671 Flagellum as a Model System for Studying Dynamic Cell-surface Events R. A. Blood- good 683 Directed Membrane Flow on the Pseudopods of Caenorhabditis elegans Spermato- zoa T. M. Roberts and S. Ward 695 Packing of Clathrin into Coats B. M. F. Pearse and R. A. Crowther 703 Surface Uptake by Fibroblasts and Its Consequences M. S. Bretscher 707 Recycling of Cell-surface Receptors: Observations from the LDL Receptor Sys- tem M. S. Brown, R. G. W. Anderson, S. K. Basu, and J. L. Goldstein 713 Functional and Structural Studies on Clathrin Triskelions and Baskets E. Unge- wickell, E. R. Unanue, and D. Branton 723 Receptor-mediated Endocytosis by Clathrin-coated Vesicles: Evidence for a Dynamic Pathway J. L. Salisbury, J. S. Condeelis, N. J. Maihle, and P. Satir 733 Microinjection of Anticlathrin Antibodies into Cultured Fibroblasts: Clathrin-coated Structures in Receptor-mediated Endocytosis and in Exocytosis J. Wehland, M. C. Willingham, M. G. Gallo, A. V. Rutherford, J. Rudick, R. B. Dickson, and I. Pastan 743 Intracellular and Extracellular Components Involved in the Formation of Ventral Surfaces of Fibroblasts W. Birchmeier, T. A. Libermann, B. A. Imhof, and T. E. Kreis 755 Cell Surface: Synthesis, Exocytosis, Endocytosis Mapping Structural Proteins of Cultured Cells by Monoclonal Antibodies J. J.-C. Lin 769 Synthesis and Assembly of the Vesicular Stomatitis Virus Glycoprotein A. Zilber- stein, M. D. Snider, and H. F. Lodish 785 The Golgi Apparatus, Coated Vesicles, and the Sorting Problem J. E. Rothman, E. Fries, W. G. Dunphy, and L. J. Urbani 797 Mechanisms for the Incorporation of Proteins into the Plasma Membrane D. Sabatini, D. Colman, E. Sabban, J. Sherman, T. Morimoto, G. Kreibich, and M. Adesnik 807 Calcium-dependent Regulation of Chromaffin Granule Movement, Membrane Contact, and Fusion during Exocytosis H. B. Pollard, C. E. Creutz, V. Fowler, J. Scott, and C. J. Pazoles 819 Are Lysosomes a Site of Enveloped-virus Penetration? M. Marsh, K. Matlin, K. Simons, H. Reggio, J. White, J. Kartenbeck, and A. Helenius 835 Cell Surface: Microvilli Structure and Function of the Brush-border Cytoskeleton P. T. Matsudaira and D. R. Burgess 845 Regulation of Contractility, Cytoskeletal Structure, and Filament Assembly in the Brush Border of Intestinal Epithelial Cells M. S. Mooseker, E. M. Bonder, B. G. Grimwade, C. L. Howe, T. C. S. Keller III, R. H. Wasserman, and K. A. Wharton 855 Characterization and Ultrastructural Role of the Major Components of the Intestinal Microvillus Cytoskeleton A. Bretscher 871 The Cytoskeleton of Intestinal Microvilli Contains Two Polypeptides Immunologically Related to Proteins of Striated Muscle E. Coudrier, H. Reggio, and D. Louvard 881 Mechanisms of Organization: Calcium Regulation and Phosphorylation of Cytoplasmic Components Multifunctional Role of Calmodulin in Biologic Processes R. W. Wallace, E. A. Tallant, and W. Y. Cheung 893 Regulation by and of Calmodulin in Mammalian Cells A. R. Means and J. G. Chaf- ouleas 903 Molecular Events in cAMP-mediated Reverse Transformation A. H. Lockwood, D. D. Trivette, and M. Pendergast 909 Regulation of Actin-Myosin Interaction by Reversible Phosphorylation of Myosin and Myosin Kinase R. S. Adeistein, M. D. Pato, J. R. Sellers, P. de Lanerolle, and M. A. Conti 921 Regulation of Myosin-filament Assembly by Light-chain Phosphorylation J. Ken- drick-Jones, P. Tooth, K. A. Taylor, and J. M. Scholey 929 Cytoskeletal Targets for Viral Transforming Proteins with Tyrosine Protein Kinase Activity B. M. Sefton, T. Hunter, E. A. Nigg, S. J. Singer, and G. Walter 939 Mechanism of Transformation by Rous Sarcoma Virus: Events within Adhesion Plaques L. Rohrschneider, M. Rosok, and K. Shrive 953 Nucleus and Cytoplasm Nuclear Lamina and the Structural Organization of the Nuclear Envelope L. Gerace and G. Blobel 967 Synthesis and Assembly of the Polypeptide Subunits of Photosystem I J. E. Mullet, A. R. Grossman, and H.-H. Chua 979 Molecular and Cellular Effects of Heat-shock and Related Treatments of Mammalian Tissue-culture Cells G. P. Thomas, W. J. Welch, M. B. Mathews, and J. R. Feramisco 985 Function of Cytoplasmic Fibers in Syncytia E. Wang, D. S. Roos, M. H. Heggeness, and P. W. Choppin 997 Cytoplasmic and Nuclear Architecture in Cells and Tissue: Form, Functions, and Mode of Assembly S. Penman, A. Fulton, D. Capco, A. Ben Ze'ev, S. Wit- telsburger, and C. F. Tse 1013 Summary Organization of the Cytoplasm B. R. Brinkley 1029
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